rabbit anti map 2 primary antibody  (Bioss)

Journal: BioMed Research International

Article Title: Bone Morphogenetic Protein-7 (BMP-7) Promotes Neuronal Differentiation of Bone Marrow Mesenchymal Stem Cells (BMSCs) In Vitro

doi: 10.1155/2021/7239783

Figure Lengend Snippet: Cell immunofluorescence detection. The expression and location of MAP-2 and Nfh were detected with the cell immunofluorescence (200x).

Article Snippet: After being treated with 0.5% Triton X-100 at room temperature for 20 min and the following washing, the cells were blocked with 5% BSA solution at room temperature for 20 min. Then, the cells were incubated with the rabbit anti-Nfh primary antibody (1 : 1500 dilution; bs-10680R; Bioss), or rabbit anti-MAP-2 primary antibody (1 : 1500 dilution; bs-1369R; Bioss), at 4°C overnight.

Techniques: Immunofluorescence, Expressing

Journal: BioMed Research International

Article Title: Bone Morphogenetic Protein-7 (BMP-7) Promotes Neuronal Differentiation of Bone Marrow Mesenchymal Stem Cells (BMSCs) In Vitro

doi: 10.1155/2021/7239783

Figure Lengend Snippet: Relative mRNA expression levels of MAP-2 and Nfh. (a, b) Quantitative real-time PCR was performed to detect the mRNA expression levels of MAP-2 (a) and Nfh (b). Compared with the control and lentiviral vector control groups, ∗ P < 0.05.

Article Snippet: After being treated with 0.5% Triton X-100 at room temperature for 20 min and the following washing, the cells were blocked with 5% BSA solution at room temperature for 20 min. Then, the cells were incubated with the rabbit anti-Nfh primary antibody (1 : 1500 dilution; bs-10680R; Bioss), or rabbit anti-MAP-2 primary antibody (1 : 1500 dilution; bs-1369R; Bioss), at 4°C overnight.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Plasmid Preparation

Journal: BioMed Research International

Article Title: Bone Morphogenetic Protein-7 (BMP-7) Promotes Neuronal Differentiation of Bone Marrow Mesenchymal Stem Cells (BMSCs) In Vitro

doi: 10.1155/2021/7239783

Figure Lengend Snippet: Relative protein expression levels of MAP-2 and Nfh. (a) Western blot analysis was performed to detect the protein expression levels of MAP-2 and Nfh. (b) Statistical analysis. Compared with the control and lentiviral vector control groups, ∗ P < 0.05.

Article Snippet: After being treated with 0.5% Triton X-100 at room temperature for 20 min and the following washing, the cells were blocked with 5% BSA solution at room temperature for 20 min. Then, the cells were incubated with the rabbit anti-Nfh primary antibody (1 : 1500 dilution; bs-10680R; Bioss), or rabbit anti-MAP-2 primary antibody (1 : 1500 dilution; bs-1369R; Bioss), at 4°C overnight.

Techniques: Expressing, Western Blot, Plasmid Preparation